Study on selective single cell isolation method for blood cells.

A LTHOUGH SOME METHODS of cell isolation for culture have been established successfully, isolation of the single cell from the peripheral blood under a high magnification has not been reported so far. Some reasons are the following: 1. Difficulties in designating the living blood cell of 15 or more under a high magnification of phase contrast microscope. 2. Technical difficulties in selectively picking up a floating desired l)lood cell. 3. Difficulty in maintaining aseptic technique. 4. Difficulty in blood cell culture especially the single cell. 5. The problem of the culture chamber. This report concerns a method for selective isolation of a single myeloblast from a patient with acute myeloblastic leukemia and its successful culture for an observation period of five days.